Mouse Primary Aortic Endothelial Cells

Authors pedro molina sánchez 1 vicente andrés 2 affiliations 1 laboratory of molecular and genetic.

Mouse primary aortic endothelial cells.

Cut off tissue to open chest and abdomen and expose aorta. Wipe off skin with 70 isopropanol and fix mice on an operation table. Caveolin 1 cav1 knockout mice were kindly provided by dr. Marek drab max planck institute for molecular cell biology and genetics dresden germany.

Enos knockout mice were kindly provided by dr. Isolation of mouse primary aortic endothelial cells by selection with specific antibodies springerlink. Mouse aortic endothelial cells were isolated from c57bl 6 mice and used for experiments without any passage. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment.

In the study of vascular biology analyses of endothelial cells ec and smooth muscle cells smc are very important. Standard biochemical procedures performed with endothelial cell cultures include rt pcr western blotting immunoprecipitation immunofluorescent staining immunofluorescent flow cytometry or generating cell derivatives for desired research applications. Paul huang cardiovascular. C57bl 6 mouse primary aortic endothelial cells can be used in assays of cell cell adhesion migration vascular tube formation or transendothelial resistance ter.

Isolation and culture of endothelial cells from mouse aorta preparation of aorta rings 1. They produce endothelium derived substances regulating vasoconstriction and vessel growth. The mouse is a critical model for research however the isolation of primary ec from murine aorta is considered difficult. Wild type c57bl 6 and p47 phox knockout mice were purchased from jackson laboratories bar harbor maine.

Primary human aortic endothelial cells acbri 375 were initiated from normal human descending aorta aortic arch to renal artery. Euthanatize mice by co 2 asphyxiation followed by cervical dislocation. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology. Mouse aortic endothelial cells maoec line the vessel wall of aorta and are constantly exposed to high hemodynamic forces.

Previously reported procedures for the isolation of ec have required magnetic beads or fluorescence activated cell sorting facs to purify the cells. Primary human aortic endothelial cells or haoecs acbri 375 were initiated from normal human descending aorta aortic arch to renal artery. The vascular endothelial cells play a significant role in many important cardiovascular disorders. Isolation of mouse primary aortic endothelial cells by selection with specific antibodies methods mol biol.

Endothelial cells ecs are key blood vessel wall components that play critical roles in the regulation of many physiological processes including angiogenesis coagulation and vascular tone.